Evaluation of Catecholamine Levels in Iraqi Female Parkinson's Patients by Using HPLC Technique

Abstract

High performance liquid chromatography Reversed–phase (Rp-HPLC) was used to estimate noradrenaline (NA), adrenaline (AD) and dopamine (DA) in the female plasma blood samples of normal volunteer's and Parkinson's patients before and after treatment with levodopa drug. Optimal condition based on ion-pairing has been performed to measure NA, AD and DA on ODS-DB column using 50 mM phosphate buffer mixed with 0.5 mM of 1- octane sulphonic acid: acetonitrile (95:5) at pH = 4.5 and monitored by UV-Visible spectrophotometer at 280 nm.
The chromatographic linearity appears to be linear in the range 0.5-10.0 ppm. The results show that the plasma concentration levels of NA, AD and DA in female normal volunteers were (236(64)), (265(84)) and (692(165)) ng/mL, respectively, whereas the plasma concentration levels of NA, AD and DA in female Parkinson's patients were (277(41)), (175(32)) and (127(42)) ng/mL before treatment with Levodopa drug using general dose of 100 mg/day. After treatment the plasma, the concentration levels become (277(37)), (214(45)) and 359(83)) ng/mL, respectively. Probably, this study has revealed that the non-enzymatic
oxidation of NA in addition to DA, promotes a toxic effect by causing an inactivation of the vesicular H+-ATPase.