Antibacterial and Antibiofilm Activity of Zinc Oxide Nanoparticles on Pseudomonas aeruginosa Isolates from Diabetic Foot Infections Grade 15

Abstract

The aim of this study was to investigate antibacterial activities and disruption of biofilm
structure by zinc oxide nanoparticles (ZnO NP). Pseudomonas aeruginosa was chosen as an
indicator of pathogenic because its natural resistance to antibiotics and its ability to form
biofilm on surfaces makes the cells impervious to therapeutic concentrations.
Twenty one isolate were taken from patients suffering from diabetic foot ulcer grads 2
infections who attended from AL-Kindy Teaching Hospital in Iraq. Different concentrations
of zinc oxide (NP) had been used (25-20000) μg/ml.
The results showed that the high concentrations (500-20000) μg /ml were lethal to bacteria also the minimum inhibitory concentration (MIC) and sub (MIC) of zinc oxide np was determined in this study. Some of the isolates were inhibit in concentration 100 μg/ml and others inhibit in concentration 75 μg/ml and according to these result the sub–MIC were 75 μg/ml to some isolates and 50 μg/ml to others, these concentration were inhibitor biofilm
production.
Our study indicates that zinc oxide nanoparticles could potentially be an antibacterial reagent to treat diseases caused by bacteria.