Spectrophotometric Determination of Sulfacetamide in Pure Form and Pharmaceutical Formulations with Metol and potassium hexacyanoferrate (ΙΙΙ)

Abstract

A simple, sensitive and accurate spectrophotometric method of determination of Sulfacetamide (SAC) in pure form and pharmaceutical formulation.
The method is based on the formation of (SAC) complex. The reaction between of the Sulfacetamide with the mixture of metol and potassium hexacyanoferrate (ΙΙΙ) was evaluated for the spectrophotometric determination of the Sulfacetamide. The maximum absorbance of the colored complex occurred at λ=540nm. Reaction conditions have been optimized to obtain (SAC) complex of high sensitivity and longer stability. Under optimum conditions the absorbance of the (SAC) complex where found to increase linearly with increase in concentration of the Sulfacetamide, which corroborated with correlation coefficient value. The concentration ranges are 0.1-100 μg ml-1 with detection limit 0.01842μg ml-1 and relative standard deviation 0.74 % and relative error of prediction for drug were lower.
The proposed method was successfully applied to determine of the selected Sulfacetamide in pure form and pharmaceutical formulations with good precision
and accuracy compared to standard method as revealed by t- and F- values and the results obtained agree well with the labeled contents.