Detection of Extended Spectrum Beta-Lactamase (ESβL) and Klebocin Production from Klebsiella pneumoniae Local Isolates from Urinary Tract Infections

Abstract

Ten isolates of Kebsiella pneumoniae were isolated from urine samples collected from patient suffering from UTI obtained from Education Labs / medical
city, and Re identification according to the biochemical tests and confirmatory by Api 20 E test. Sensitivity of isolates was tested against 23 Antibiotics, results
revealed that isolates showed multi resistance to antibiotics ranging between (7-19) antibiotics, and all isolates of K.pneumoniae were resistant 100% to Ampicillin, Cephalexin, Amoxicillin, ,Penicillin ,Tetracyclin, Gentamycin and Amikacin, in addition the isolates showed high resistance to third generation of cephalosporines included (60)% resistance to ceftazidime and (80)% resistance to cefotaxime, and ceftriaxone . Imipenem and azteronem was found to be the most effective agents against the isolates.
Detection of Beta–Lactamase by using rapid standard Iodometric assay and capillary tubes method, showed that 50% (5 isolates) of K.pneumoniae were Blactamase producing All β- lactamase- producing K.pneumoniae isolates were also tested for their ability to produce Extended-Spectrum Beta-lactamases (ESBLs), screening and confirmatory tests recommended by NCCLS it considered the most accurate method for detection of ESBL- producing isolates.

Determination the MICs of ESBL-producer isolates against some β-lactam antibiotics showed that five ESBL-producing K.pneumoniae isolates was highly
resistant for both ampicillin and amoxicillin. They was also resistant to ceftazidime, cefotaxime, and ceftriaxone high MIC results from (32to >128) (32to>256)
(32to>128) μg/ml respectively.
Results of detection of klebocin production by klebsiella isolates showed that 20 % of them were klebocin-producers against some indicator isolates of G- and
G+ bacteria.
Patterns of plasmid was studies of selected isolate K. pneumoniae 6, showed its harbor two plasmid bands different in size and position.
Attempts to cure plasmids was unsuccessful from the selected ESBLs and klebsin producer isolate ( K.pneumoniase 6 ), in concentration ranged from (10-
100) μg/ml when we used mitomycin – C.
Results of conjugation experiment revealed that genes encoding for the production of BLs, and resistance to penicillin, ampicillin, amoxicillin, cefotaxime,
ceftraxione and ceftazidime, were located on plasmids.