Kinetic Studies and electrophoretic separation of Alkaline Phosphatase Isoenzyme from Cancerous patients
The activity of sALP was determined by the calorimetric method King_Armistrong in serum of 303 cancerous patients and 42 normal healthy controls. The results show that the ALP activity was increased in all types of cancer tissues, however ALP activity show a significant increase only in bone, liver and pancreas tissues. The increase of ALP activity could be used as tumor marker for bone, liver and pancreas tissues.
The increase in sALP activity could be used as tumor marker for bon and liver cancer and to detect metastasis to the organ. No significant differences of sALP activity were found between males and females. The activity was increase with the stage of development of the disease.
The kinetic studies for normal healthy human were measured at a substrate concentration 10 mM, Km 2.7 mM, temperature 370c and pH 10. The melectrophoretic studies show that one band of ALP in serum of normal subjects, as compared to cancerous patients where several bands were detected in cancer patients; the intensity of band color varied with type; age and stage of disease