Binding of 99mTc to human serum albumin

Abstract

The specificity of ^99m Tc-MDP binding to HSA was examined , the results showed that the non specific binding was 21-23% of the total binding . The range of optimum pH for the binding was 7.4-7.6 . The most suitable amounts for HSA to give maximum binding was (70mg)per incubation medium , binding seemed to be affected by ^99m Tc- MDP concentration and also mono and divalent metal chloride salts. The association constants (ka) and maximum binding capacity (B_max) of ^99m Tc-MDP binding to its binding sites of HSA was determined from scatchard and double reciprocal plots , the results revealed that (ka) and (B_max) values were temperature dependant.