DetectionOf genesResponsible for BiofilmsFormedby Klebsiella pneumoniaeandEsherichia coliand their effect on innate immunity

Abstract

Biofilms as a major virulence factor of bacteria ,therelationship between bacteria persistence in the urinary tract and genes responsible for biofilms production was studied. The aim is to detect the presence,frequency of  the,fimA, fimH, mrkA  and  mrkD, genes  and biofilms production in two species isolated from UTI patients and their effect on some innate immunity aspects. Sixty five isolates of E.coli  and fifty of K.pneumoniae isolates were collected. All isolates were initially diagnosed  as genus Esherichiacoli andKlebsiella pneumonia. Final identification for the isolates was done by Vitek 2 compact system. The ability to form biofilm was carried out by using Tissue culture plate method (TCP).Adhesion average to epithelial cells of E.coli and K.pneumoniae was studied. Detection of genes were done to   by using PCR technique . Immunological experiments were done by using bactericidal activity and opsonization factor. UTI infection were more common in female than male. 75% (49/65) females, 25%(16/65)  males ofE.coli isolates and 88% (44/50) females,12%(6/50) males of K.pneumoniaeisolates. Patients ages in children between (2 months -18years) in UTI  caused by E.coli was 35%(23/65) while in k.pneumoniae was 74%(37/50),Ages between (18 years-50 years) in E.coli was 37%(24/65) while in K.pneumoniae was 18%(9/50) finally,Ages between (50 years-72years) in E.coli was 28%(18/65) while in K.pneumoniae was 8%(4/50).69% of E.coli isolates and 72% of k.pneumoniae isolates were higher biofilm producers. . Adhesion average to epithial cells of E.coli and K.pneumoniae was 42.9% and 44% respectively. Both E.coli and K.pneumoniae showed that 100% 0f the 12 isolate harbored the fimA, fimH, mrkA and mrkD genes.  The bactericidal average was higher in E.coli than K.pneumoniae  , P- value( 0.01)  for mice injected with fimbriae,  Opsonization  factor  was higher in E.coli than K.pneumoniae the P value (0.05) .                           Conclusion: A strong relationship between biofilms production and presence of genes of different species isolated from same clinical source that has effected on resistance of bacteria to innate immunity.