Antioxidative effect of metformin on valproic acid induced hepatoxicity in male rats

Intesar Tarik Numan; Nadia Hameed Mohamed; Zainab Khalid Ali

doi:10.32947/ajps.v22i3.885

Authors

Intesar Tarik Numan Department of Pharmacology and Toxicology, AL-Huda University College, AL Anbar, iraq
Nadia Hameed Mohamed Department of Clinical Laboratory Science, College of Pharmacy, Mustansiriyah University
Zainab Khalid Ali Department of Pharmacology and Toxicology, College of Pharmacy, Mustansiriyah University

DOI:

https://doi.org/10.32947/ajps.v22i3.885

Abstract

Metformin is 1,1-dimethylbiguanide hydrochloride, is the first-line therapy for type 2 diabetes. Additionally, several studies focused on the role of metformin in antioxidant activities for the treatment of hepatic disorders. The experimentally

-based result on valproic acid's liver injury, a front-line medicine for the treatment of epilepsy, attracted a lot of interest. As a result, the effect of metformin on valproic acid-induced redox disturbances in rat hepatic tissue was studied. metformin at 250 mg/kg dose was administered via oral gavage for 30 days, and valproic acid at a dose of 400 mg/kg was administered by intraperitoneal route starting from the twenty-second day of the experiment, for eight days to induce hepatotoxicity. Treatment with metformin reduced valproic acid-enhancing alanine aminotransferase, aspartate aminotransferase activities. Tissue levels of malondialdehyde in the liver tissue of valproic acid-treated rats significantly increased (P-value < 0.05) whereas glutathione decreased. The coadministration of metformin with valproic acid significantly decreased the malondialdehyde levels and increased glutathione levels (P-value < 0.05). Finally, metformin protected rats from valproic acid-induced hepatotoxicity, improved antioxidant status, and reduced hepatic oxidative stress.

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