Diagnosis of Enteroviral-associated-Meningitis by RT-PCR for the First Time in Iraq

Faiza L. Tuama; Yassen I. Omeran; Manal Khalid Abdul Ridha; Walid K. Ridha

doi:10.32947/ajps.v5i1.388

Diagnosis of Enteroviral-associated-Meningitis by RT-PCR for the First Time in Iraq

Authors

Faiza L. Tuama Central Public Health Laboratory/Molecular Biology Department
Yassen I. Omeran Al-Nahrain university/Collage of Science/ Biotechnology Department
Manal Khalid Abdul Ridha College of Pharmacy, Pharmacotheraputics dept
Walid K. Ridha Central Public Health Laboratory/Virology Department.

DOI:

https://doi.org/10.32947/ajps.v5i1.388

Keywords:

Enteroviral-associated- meningitis, EV-RT-PCR

Abstract

Introduction: A protocol for testing cerebrospinal fluid specimens using RT-PCR for the diagnosis of central nervous system infections was developed 2 and used to test 20 CSF specimens.
Objectives: A-Evaluation of efficiency of In-house prepared Extraction reagent. BAmplified of Enteroviral-RNA genome by single-step RT-PCR. Methods: 20
cerebrospinal fluid specimens were included. RNA was extracted from 250μl by method Chomcynki, 1993. EV-RT-PCR was performed for all specimens studied.
Results: We successfully isolated total RNA from 20 CSF specimens of AM cases. Ten of 20 specimens were positive by RT-PCR.

Conclusions: Single-step extraction method was allowed isolation of RNA in 4hr. and provided both high yield and purity of un-degraded RNA preparation. EV-RTPCR detection test is suitable for clinical diagnosis of EV-related meningitis and may improve the management of EV-related neurologic syndromes.

Downloads

Published

2008-06-01

How to Cite

Tuama, F. L., Omeran, Y. I., Abdul Ridha, M. K., & Ridha, W. K. (2008). Diagnosis of Enteroviral-associated-Meningitis by RT-PCR for the First Time in Iraq. Al Mustansiriyah Journal of Pharmaceutical Sciences, 5(1), 35–43. https://doi.org/10.32947/ajps.v5i1.388

Issue

Vol. 5 No. 1 (2008): volume 5,Issue 1. 2008

Section

Articles

License

AJPS is an open-access journal that all contents are free of charge. Articles of this journal are licensed under the terms of the Creative Commons Attribution International Public License CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/legalcode) that licensees are unrestrictly allowed to search, download, share, distribute, print, or link to the full texts of the articles, crawl them for indexing and reproduce any medium of the articles provided that they give the author(s) proper credits (citation). The journal allows the author(s) to retain the copyright of their published article.

Creative Commons-Attribution (BY)