Detection of Inhibitory Effect of Aluminium Potassium Sulfate Against Some Bacterial and Fungal Strains

Afrah M H Salman; Fitua Al –Saedi; Hussien Ali Karim

doi:10.32947/ajps.v25i5.1285

Authors

Afrah M H Salman Department of toxicology and pharmacology, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq
Fitua Al –Saedi Department of Clinical Laboratory Sciences, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq
Hussien Ali Karim Department of pharmaceutical chemistry, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq

DOI:

https://doi.org/10.32947/ajps.v25i5.1285

Keywords:

Aluminium potassium sulfate, antibacterial activity, antifungal activity, bacterial resistance, natural products

Abstract

The percentage of microbial resistance to antibiotics has been remarkably increasing in the last decades. For this reason, there has been an increasing demand for using natural products molecules for medicinal purposes. The current study revealed the antimicrobial activity of aluminium potassium sulfate (alum) against the selected pathogens which includes gram positive and negative bacteria and fungus.Alum inhibits the growth of Pseudomonas aeruginosa and Staphylococcus aureus at all concentrations (50,40,30,20, 10 and 5 %).

The percentage of microbial resistance to antibiotics has been remarkably increasing in the last decades. For this reason, there has been an increasing demand for using natural products molecules for medicinal purposes. The current study revealed the antimicrobial activity of aluminium potassium sulfate (alum) against the selected pathogens which includes gram positive and negative bacteria and fungus.Alum inhibits the growth of Pseudomonas aeruginosa and Staphylococcus aureus at all concentrations (50,40,30,20, 10 and 5 %). However, Vibrio cholera growth was inhibited at the concentrations of (50, 40, 30, 20 and 10 %). The antimicrobial activity of alum against the selected pathogenic fungi (Cryptococcus neoformance , Cryptococcus gattii, Rhizopus micrococcus and Mucor circinelloiles) was measured. The results showed that alum has excellent antimicrobial activity against all the selected pathogenic fungi in all concentrations. With no inhibitory effect against both Rhizopus micrococcus and Mucor circinelloides at the lowest concentration of alum (5%). The growth of fungi is significantly reduced by 4-fold in the presence of alum in comparison with those grown in YPD media alone. Which means that half of antimicrobial activity of alum is due to inhibitory mechanism differ from the acidification. The above results confirmed the antimicrobial activity of alum against the selected pathogens, and proved that the inhibitory activity of alum is not only due to the low pH produced by alum but also to the presence of other inhibitory mechanism of alum. Regarding these results further study is needed to understand the inhibitory mechanisms of alum with neglecting the acidity effect of alum.

References

1- Abdulwahab AM, Al-magdashi YA, Meftah A, Al-Eryani DA, Qaid AA. Growth, structure, thermal, electrical and optical properties of potassium aluminum sulfate dodecahydrate (potash alum) single crystal. Chinese Journal of Physics. 2019 Aug 1; 60:510-21.

2- Dhingra S, Rahman NA, Peile E, Rahman M, Sartelli M, Hassali MA, Islam T, Islam S, Haque M. Microbial resistance movements: an overview of global public health threats posed by antimicrobial resistance, and how best to counter. Frontiers in Public Health. 2020 Nov 4; 8:535668.

3- Polash SA, Varadi L, Shukla R. Microbial Resistance Mechanisms and Potential of Metal-Organic Framework in Mitigation Thereof. InNano-Strategies for Addressing Antimicrobial Resistance: Nano-Diagnostics, Nano-Carriers, and Nano-Antimicrobials 2022 Dec 18 (pp. 237-277). Cham: Springer International Publishing.

4- Mao J, Guan S, Chen Y, Zeb A, Sun Q, Lu R, Dong J, Wang J, Cao D. Application of a deep generative model produces novel and diverse functional peptides against microbial resistance. Computational and Structural Biotechnology Journal. 2023 Jan 1; 21:463-71.

5- Al-Saedi, F. Strategies in anti-adhesion therapy: A review article. Al Mustansiriyah Journal of Pharmaceutical Sciences, 2021; 21(1). Genilloud O. Natural products discovery and potential for new antibiotics. Current opinion in microbiology. 2019 Oct 1; 51:81-7.

6- Vignesh K, Niresh SJ, Saravanasingh K, Uma AP. Padikaram (alum)–a unique drug and its utilization in Siddha medicine: a pharmacological review. Siddha Papers. 2019;14(2):1-2.

7- Sasaki E, Asanuma H, Momose H, Furuhata K, Mizukami T, Hamaguchi I. Nasal alum-adjuvanted vaccine promotes IL-33 release from alveolar epithelial cells that elicits IgA production via type 2 immune responses. PLoS Pathogens. 2021 Aug 30;17(8):e1009890.

8- Valgas C, Souza SM, Smânia EF, Smânia Jr A. Screening methods to determine antibacterial activity of natural products. Brazilian journal of microbiology. 2007; 38:369-80.

9- Khalid K. Al-Bayatti, Fitua, M. Aziz, Mayssaa E. Abdalah. A Study of Antibacterial Activity of Cidar (Zizyphus spinachristi L.) on Bacterial Pathogens isolated from Skin Infections. Al Mustansiriyah Journal of Pharmaceutical Sciences. 2011. 9(1), 13-20.

10- Shahriari R, Salari S, Shahriari S. In vitro study of concentration-effect and time-course pattern of white alum on Escherichia coli O157: H7 growth. African Journal of Traditional, Complementary and Alternative Medicines. 2017 Feb 22;14(2):311-8.

11- Amadi LO. A review of antimicrobial properties of alum and sundry applications. Acta Scientific Microbiology. 2020;3(4):109-17.

12- Mahalaxmi I, Jayaramayya K, Venkatesan D, Subramaniam MD, Renu K, Vijayakumar P, Narayanasamy A, Gopalakrishnan AV, Kumar NS, Sivaprakash P, Rao KR. Mucormycosis: An opportunistic pathogen during COVID-19. Environmental research. 2021 Oct 1; 201:111643.

13- León-Buitimea A, Garza-Cervantes JA, Gallegos-Alvarado DY, Osorio-Concepción M, Morones-Ramírez JR. Nanomaterial-based antifungal therapies to combat fungal diseases aspergillosis, Coccidioidomycosis, Mucormycosis, and candidiasis. Pathogens. 2021 Oct;10(10):1303.

14- Diniz-Lima I, Fonseca LM, Silva-Junior EB, Guimarães-de-Oliveira JC, Freire-de-Lima L, Nascimento DO, Morrot A, Previato JO, Mendonça-Previato L, Decote-Ricardo D, Freire-de-Lima CG. Cryptococcus: History, Epidemiology and Immune Evasion. Applied Sciences. 2022 Jul 13;12(14):7086.

15- Hsiao PJ, Cheng H, Kao YH, Wang YH, Chiu CC, Chiang WF, Kuo CC, Chuu CP, Wu KA. Comparison of laboratory diagnosis, clinical manifestation, and management of pulmonary cryptococcosis: Report of the clinical scenario and literature review. Clinica Chimica Acta. 2022 Jan 1; 524:78-83.

16- Amich J, Vicentefranqueira R, Leal F, Calera JA. Aspergillus fumigatus survival in alkaline and extreme zinc-limiting environments relies on the induction of a zinc homeostasis system encoded by the zrfC and aspf2 genes. Eukaryotic cell. 2010 Mar;9(3):424-37.

17- Plüddemann A, Mukhopadhyay S, Gordon S. Innate immunity to intracellular pathogens: macrophage receptors and responses to microbial entry. Immunological reviews. 2011 Mar;240(1):11-24.

18- Mesquita LA, Bailão AM, de Curcio JS, da Silva KL, da Rocha Fernandes G, Silva-Bailão MG, Novaes E, de Almeida Soares CM. Global Molecular Response of Paracoccidioides brasiliensis to Zinc Deprivation: Analyses at Transcript, Protein and MicroRNA Levels. Journal of Fungi. 2023 Feb 21;9(3):281.

19- Butler J, Handy RD, Upton M, Besinis A. Review of Antimicrobial Nanocoatings in Medicine and Dentistry: Mechanisms of Action, Biocompatibility Performance, Safety, and Benefits Compared to Antibiotics. ACS nano. 2023 Apr 7;17(8):7064-92.

20- Ngolong Ngea GL, Qian X, Yang Q, Dhanasekaran S, Ianiri G, Ballester AR, Zhang X, Castoria R, Zhang H. Securing fruit production: Opportunities from the elucidation of the molecular mechanisms of postharvest fungal infections. Comprehensive reviews in food science and food safety. 2021 May;20(3):2508-33.