Preparation and Evaluation of Hydrogel Containing Prednisolone Nanoparticles

Authors

  • Walaa A. Saihood College of Pharmacy, Department of Pharmaceutics, AL-Mustansiriyah University
  • Hayder K. Abbas Department of Pharmaceutics, College of Pharmacy, University of Alkafeel

DOI:

https://doi.org/10.32947/ajps.v23i4.1101

Keywords:

nanoparticle, prednisolone, nanosuspension, particle size, hydrogel

Abstract

Currently, nanoparticles technology is well known and it used to augment the rate of drug dissolution and considerably increased bioavailability. Nanoparticles have been combined with hydrogel to form nanoparticle-hydrogel hybrid system as a substitute to conventional dosage forms.

The goal of this study is to prepare an effective hydrogel form containing prednisolone nanoparticles as a hybrid biomaterial system for transdermal application. Six hydrogel formulations containing prednisolone nanoparticles were prepared and characterized for particle size, entrapment efficiency, physical appearance, pH, drug content, spreadability test, viscosity, in-vitro drug release and ex-vivo diffusion. The results showed that the all preparations in the nanometer size and the drug content of prednisolone in hydrogel was ranged from 96.24% to 99.15% and there was a significant enhancement (p<0.05) in drug release and permeation from the prepared nanoparticles -hydrogel formulas when compared with plain hydrogel of prednisolone. From this study, it can be concluded that nanoparticle-hydrogel system could be a possible drug delivery technology for transdermal application of prednisolone drug.

References

- Kumar A, Chen F, Mozhi A, Zhang X, Zhao Y, Xue X, Hao Y, Zhang X, Wang PC, Liang XJ. Innovative pharmaceutical development based on unique properties of nanoscale delivery formulation. Nanoscale. 2013;5(18):8307-25. DOI: https://doi.org/10.1039/c3nr01525d

- Ehmann F, Sakai-Kato K, Duncan R, Pérez de la Ossa DH, Pita R, Vidal JM, Kohli A, Tothfalusi L, Sanh A, Tinton S, Robert JL. Next-generation nanomedicines and nanosimilars: EU regulators’ initiatives relating to the development and evaluation of nanomedicines. Nanomedicine. 2013 May;8(5):849-56. DOI: https://doi.org/10.2217/nnm.13.68

- Müller RH, Gohla S, Keck CM. State of the art of nanocrystals–special features, production, nanotoxicology aspects and intracellular delivery. European journal of pharmaceutics and biopharmaceutics. 2011 May 1;78(1):1-9. DOI: https://doi.org/10.1016/j.ejpb.2011.01.007

- Palanisamy M, Khanam J. Solid dispersion of prednisolone: solid state characterization and improvement of dissolution profile. Drug development and industrial pharmacy. 2011 Apr 1;37(4):373-86. DOI: https://doi.org/10.3109/03639045.2010.513984

- Zakeri-Milani P, Nezhadi SH, Barzegar-Jalali M, Mohammadi L, Nokhodchi A, Valizadeh H. Studies on dissolution enhancement of prednisolone, a poorly water-soluble drug by solid dispersion technique. Advanced Pharmaceutical Bulletin. 2011 Jun;1(1):48.

- Basha BN, Prakasam K, Goli D. Formulation and evaluation of gel containing fluconazole-antifungal agent. International Journal of Drug Development and Research. 2011;3(4):109-112.

- McKenzie M, Betts D, Suh A, Bui K, Kim LD, Cho H. Hydrogel-based drug delivery systems for poorly water-soluble drugs. Molecules. 2015 Nov 13;20(11):20397-408. DOI: https://doi.org/10.3390/molecules201119705

- Gao W, Vecchio D, Li J, Zhu J, Zhang Q, Fu V, Li J, Thamphiwatana S, Lu D, Zhang L. Hydrogel containing nanoparticle-stabilized liposomes for topical antimicrobial delivery. Acs Nano. 2014 Mar 25;8(3):2900-7. DOI: https://doi.org/10.1021/nn500110a

- Thomas V, Yallapu MM, Sreedhar B, Bajpai SK. Breathing‐in/breathing‐out approach to preparing nanosilver‐loaded hydrogels: Highly efficient antibacterial nanocomposites. Journal of Applied Polymer Science. 2009 Jan 15;111(2):934-44. DOI: https://doi.org/10.1002/app.29018

- Merino S, Martin C, Kostarelos K, Prato M, Vázquez E. Nanocomposite hydrogels: 3D polymer–nanoparticle synergies for on-demand drug delivery. ACS nano. 2015 May 26;9(5):4686-97. DOI: https://doi.org/10.1021/acsnano.5b01433

- Yadav PS, Kumar V, Singh UP, Bhat HR, Mazumder B. Physicochemical characterization and in vitro dissolution studies of solid dispersions of ketoprofen with PVP K30 and d-mannitol. Saudi Pharmaceutical Journal. 2013 Jan 1;21(1):77-84. DOI: https://doi.org/10.1016/j.jsps.2011.12.007

- Nunes D, Andrade S, Ramalho MJ, Loureiro JA, Pereira MC. Polymeric nanoparticles-loaded hydrogels for biomedical applications: a systematic review on in vivo findings. Polymers. 2022 Mar 2;14(5):1010. DOI: https://doi.org/10.3390/polym14051010

- Moorthi C, Kathiresan K. Fabrication of highly stable sonication assisted curcumin nanocrystals by nanoprecipitation method. Drug Invention Today. 2013 Mar 1;5(1):66-9. DOI: https://doi.org/10.1016/j.dit.2013.02.003

- Kim BS, Won M, Yang, Lee KM, Kim CS. In vitro permeation studies of nanoemulsions containing ketoprofen as a model drug. Drug Delivery. 2008 Jan 1;15(7):465-9.

- Speedy L. The effects of shear on neutralized carbomers in aqueous conditions. Monash University, Australia, semanticscholar. org. 2014

- Kim BS, Won M, Yang, Lee KM, Kim CS. In vitro permeation studies of nanoemulsions containing ketoprofen as a model drug. Drug Delivery. 2008 Jan 1;15(7):465-9. DOI: https://doi.org/10.1080/10717540802328599

- Arora R, Aggarwal G, Harikumar SL, Kaur K. Nanoemulsion based hydrogel for enhanced transdermal delivery of ketoprofen. Advances in Pharmaceutics. 2014 Dec 16;2014. DOI: https://doi.org/10.1155/2014/468456

- Helal DA, El-Rhman DA, Abdel-Halim SA, El-Nabarawi MA. Formulation and evaluation of fluconazole topical gel. Int J Pharm Pharm Sci. 2012;4(5):176-83.

- Suksaeree J, Monton C, Sakunpak A, Charoonratana T. Formulation and in vitro study of ketoprofen pseudolatex gel for transdermal drug delivery systems. International Journal of Pharmacy and Pharmaceutical Sciences. 2014;6(2):248-53.

- Mowafaq M. Ghareeb. Formulation and characterization of rizatriptan benzoate as intranasal nanoemulgel. Int. J. Drug Deliv. Technol. 2022;12, 546-552.

- Ueda CT, Shah VP, Derdzinski K, Ewing G, Flynn G, Maibach H, Marques M, Rytting H, Shaw S, Thakker K, Yacobi A. Topical and transdermal drug products. InPharmacopeial forum 2009 May (Vol. 35, No. 3, pp. 750-764).

- Hayder K. Abbas, Inas F. AbdulRazzaq, Yasmien A. Salal, Dhurgham Q. Shaheed. Preparation and evaluation of proniosomal form containing diphenhydramine HCl. Drug Invention Today.2020 May13(2):313-323.

- Samia O, Hanan R, Kamal ET. Carbamazepine mucoadhesive nanoemulgel (MNEG) as brain targeting delivery system via the olfactory mucosa. Drug delivery. 2012 Jan 1;19(1):58-67. DOI: https://doi.org/10.3109/10717544.2011.644349

- Bayanati M, Khosroshahi AG, Alvandi M, Mahboobian MM. Fabrication of a thermosensitive in situ gel nanoemulsion for nose to brain delivery of temozolomide. Journal of Nanomaterials. 2021 Mar 13;2021. DOI: https://doi.org/10.1155/2021/1546798

- Zhao X, Liu JP, Zhang X, Li Y. Enhancement of transdermal delivery of theophylline using microemulsion vehicle. International journal of pharmaceutics. 2006 Dec 11;327(1-2):58-64. DOI: https://doi.org/10.1016/j.ijpharm.2006.07.027

- Mostafa DM, Abd El-Alim SH, Asfour MH, Al-Okbi SY, Mohamed DA, Hamed TE, Awad G. Transdermal fennel essential oil nanoemulsions with promising hepatic dysfunction healing effect: in vitro and in vivo study. Pharmaceutical development and technology. 2019 Jul 3;24(6):729-38. DOI: https://doi.org/10.1080/10837450.2019.1584633

- Kaur R, Ajitha M. Transdermal delivery of fluvastatin loaded nanoemulsion gel: Preparation, characterization and in vivo anti-osteoporosis activity. European journal of pharmaceutical sciences. 2019 Aug 1;136:104956. DOI: https://doi.org/10.1016/j.ejps.2019.104956

- Laothaweerungsawat N, Neimkhum W, Anuchapreeda S, Sirithunyalug J, Chaiyana W. Transdermal delivery enhancement of carvacrol from Origanum vulgare L. essential oil by microemulsion. International Journal of Pharmaceutics. 2020 Apr 15;579:119052. DOI: https://doi.org/10.1016/j.ijpharm.2020.119052

- Abbas HK, Bader QA, Hussein AH, Shaheed DQ. Preparation and in vitro evaluation of clove oil microemulsion. Int. J. Pharm. Res. 2020 Apr;12:422-30. DOI: https://doi.org/10.31838/ijpr/2020.12.03.065

- El-Badry12 M, Fetih G, Salem-Bekhit MM, Shakeel F. Formulation and evaluation of nanosuspension of albendazole for dissolution enhancement. Nanoscience and Nanotechnology letters. 2013;5(9):1024-9. DOI: https://doi.org/10.1166/nnl.2013.1645

- Dong Y, Ng WK, Shen S, Kim S, Tan RB. Preparation and characterization of spironolactone nanoparticles by antisolvent precipitation. International journal of pharmaceutics. 2009 Jun 22;375(1-2):84-8. DOI: https://doi.org/10.1016/j.ijpharm.2009.03.013

- Shekunov BY, Baldyga J, York P. Particle formation by mixing with supercritical antisolvent at high Reynolds numbers. Chemical Engineering Science. 2001 Apr 1;56(7):2421-33. DOI: https://doi.org/10.1016/S0009-2509(00)00443-7

- Lukić M, Pantelić I, Savić SD. Towards optimal ph of the skin and topical formulations: From the current state of the art to tailored products. Cosmetics. 2021 Aug 4;8(3):69. DOI: https://doi.org/10.3390/cosmetics8030069

- Kumar L, Verma R. In vitro evaluation of topical gel prepared using natural polymer. International journal of drug delivery. 2010 Jan 1;2(1). DOI: https://doi.org/10.5138/ijdd.2010.0975.0215.02012

- Jelvehgari M, Montazam H. Evaluation of mechanical and rheological properties of metronidazole gel as local delivery system. Archives of pharmacal research. 2011 Jun;34(6):931-40. DOI: https://doi.org/10.1007/s12272-011-0610-5

- El-Kased RF, Amer RI, Attia D, Elmazar MM. Honey-based hydrogel: In vitro and comparative In vivo evaluation for burn wound healing. Scientific reports. 2017 Aug 29;7(1):9692. DOI: https://doi.org/10.1038/s41598-017-08771-8

- Baby DK. Rheology of hydrogels. InRheology of Polymer Blends and Nanocomposites 2020 Jan 1 (pp. 193-204). Elsevier. DOI: https://doi.org/10.1016/B978-0-12-816957-5.00009-4

- Zakaria AS, Afifi SA, Elkhodairy KA. Newly developed topical cefotaxime sodium hydrogels: antibacterial activity and in vivo evaluation. BioMed research international. 2016 Jan 1;2016. DOI: https://doi.org/10.1155/2016/6525163

- Noor AH, Ghareeb MM. Formulation and evaluation of ondansetron HCl nanoparticles for transdermal delivery. Iraqi Journal of Pharmaceutical Sciences (P-ISSN 1683-3597 E-ISSN 2521-3512). 2020 Dec 27;29(2):70-9. DOI: https://doi.org/10.31351/vol29iss2pp70-79

- El Maghraby GM. Transdermal delivery of hydrocortisone from eucalyptus oil microemulsion: effects of cosurfactants. International Journal of Pharmaceutics. 2008 May 1;355(1-2):285-92. DOI: https://doi.org/10.1016/j.ijpharm.2007.12.022

- AlEbadi NN, Al-Lami MS. Formulation and in-vitro Evaluation of Ethosomes using Anastrozole as a Modeling Drug. Al Mustansiriyah Journal of Pharmaceutical Sciences. 2022;22(4):90-105. DOI: https://doi.org/10.32947/ajps.v22i4.971

- Ali MH, Ali WK. Preparation and Evaluation of Emulgel as Topical Drug Delivery for Nimesulide by Using Conventional Emulsion. Al Mustansiriyah Journal of Pharmaceutical Sciences. 2019 Dec 1;19(4):16-26. DOI: https://doi.org/10.32947/ajps.v19i4.629

Published

2023-10-09

How to Cite

Walaa A. Saihood, & Hayder K. Abbas. (2023). Preparation and Evaluation of Hydrogel Containing Prednisolone Nanoparticles. Al Mustansiriyah Journal of Pharmaceutical Sciences, 23(4), 463–476. https://doi.org/10.32947/ajps.v23i4.1101