Quercetin Improves the Histopathological Changes of Liver Caused by Interferon Beta 1b-induced Liver Injury
DOI:
https://doi.org/10.32947/ajps.v25i2.1153Keywords:
Quercetin, interferon beta 1b(betaferon), liver injury, multiple sclerosis (MS)Abstract
Betaferon or interferon beta-1b (INFβ-1b) is a drug that used for multiple sclerosis (MS) treatment, this drug demonstrated an efficacy in relapse MS, but it can cause hepatotoxicity or liver injury, which present as brief elevations in liver aminotransferase enzymes. Quercetin which acts as antioxidant, anti-apoptotic and anti-inflammatory was used as a hepatoprotective in the current study.
This study aimed to investigate the histopathological changes of the liver tissue by betaferon and study the effect of different doses of quercetin on the liver pathological changes.
Methodology: (36) male rats were divided into 6 groups include the negative control group (given only normal saline i.p), betaferon or induction group (given only betaferon 250µg/kg i.p), treatment groups (quercetin 25,50,100 mg/kg + betaferon 250µg/kg) and silymarin group (silymarin 100 mg/kg i.p). In the current study, liver histopathological changes were examined by Hematoxylin and Eosin staining.
Results: In the control group the histological appearance of the liver showed normal tissues (no histopathological changes). Betaferon group the histopathological appearance of the liver showed sever hepatitis with multiple focal necrosis, sever dilation of central vein and marked cellular swelling (Ballooning cells) of hepatocytes. Histopathological examination of the groups pretreated with different doses of quercetin reflected dose-dependent hepatoprotective effects. Pretreatment with (25mg/kg) quercetin mildly improved the histopathological appearance of the liver. When the dose was increased to (50mg/kg), the score improved additionally, with the hepatic injury resolving at (100 mg/kg) of quercetin pretreatment as in silymarin group. Conclusions: Quercetin can improve histopathological changes on the tissue of the liver of male rats indicates that it possesses advantageous properties in reducing the histopathological damage induced by betaferon.
References
1- Filipi, Mary, and Samantha Jack. “Interferons in the Treatment of Multiple Sclerosis: A Clinical Efficacy, Safety, and Tolerability Update.” International journal of MS care vol. 22,4 (2020): 165-172.
2- Bergamaschi, Roberto, et al. "Immunomodulatory therapies delay disease progression in multiple sclerosis." Multiple Sclerosis Journal 22.13 (2016): 1732-1740.
3- Hamdan, Sarah Saad, Yassir Mustafa Kamal, and Huda Jaber Waheed. "Astaxanthin effect on apoptotic biomarkers in methotrexate-induced liver injury." Al Mustansiriyah Journal of Pharmaceutical Sciences 22.3 (2022): 43-50.
4- John P. Cunha, DO, FACOEP.betaseron. Last updated on RxList: 9/20/2022.
5- Hervás-García, J. V., et al. "Toxic hepatitis after concomitant interferon beta and aloe vera treatment in a patient with multiple sclerosis: A case report." Neurologia (Barcelona, Spain) 32.8 (2016): 546-547.
6- Balasa, Rodica et al. “Reviewing the Significance of Blood-Brain Barrier Disruption in Multiple Sclerosis Pathology and Treatment.” International journal of molecular sciences vol. 22,16 8370. 4 Aug. 2021.
7- LiverTox: Clinical and Research Information on Drug-Induced Liver Injury [Internet]. Bethesda (MD): National Institute of Diabetes and Digestive and Kidney Diseases; 2012-. Autoimmune Hepatitis.
8- Saeedi M, Forughipour M, Sasannezhad P, Shoeibi A. Interferon-Beta-1b Induced Autoimmune Hemolytic Anemia in a Patient with MS: A Case Report. Iran Red Crescent Med J. 2011 Mar;13(3):210-2. Epub 2011 Mar 1.
9- Guy, Jennifer, and Marion G Peters. “Liver disease in women: the influence of gender on epidemiology, natural history, and patient outcomes.
10- Kelly, Gregory S. "Quercetin." Alternative medicine review 16.2 (2011): 172-195.
11- Lakhanpal, Parul, and Deepak Kumar Rai. "Quercetin: a versatile flavonoid." Internet Journal of Medical Update 2.2 (2007): 22-37.
12- Chen, Li, et al. "Quercetin and non-alcoholic fatty liver disease: A review based on experimental data and bioinformatic analysis." Food and Chemical Toxicology 154 (2021): 112314.
13- Liu, Hui, Lei Zhang, and Shaoping Lu. "Evaluation of antioxidant and immunity activities of quercetin in isoproterenol-treated rats." Molecules 17.4 (2012): 4281-4291.
14- Costa, Lucio G., et al. "Mechanisms of neuroprotection by quercetin: counteracting oxidative stress and more." Oxidative medicine and cellular longevity 2016 (2016).
15- Heidarian, Esfandiar, and Ali Nouri. "Hepatoprotective effects of silymarin against diclofenac-induced liver toxicity in male rats based on biochemical parameters and histological study." Archives of Physiology and Biochemistry 127.2 (2021): 112-118.
16- Anthony L. Junqueira’s Basic Histology text and atlas. Edition. McGraw-Hill Education. 2016.
17- Alempijevic, Tamara et al. “Drug-induced liver injury: Do we know everything?” World journal of hepatology vol. 9,10 (2017): 491-502.
18- Arif, Inam Sameh, et al. "Role of miRNA in drug-induced hepatic injury." AJPS (2022): 1.
19- Villani, Rosanna et al. “Autoimmune liver disease and multiple sclerosis: state of the art and future perspectives.” Clinical and experimental medicine vol. 23,7 (2023): 3321-3338.
20- Rafiee Zadeh, Aryan et al. “Mechanism and adverse effects of multiple sclerosis drugs: a review article. Part 1.” International journal of physiology, pathophysiology, and pharmacology vol. 11,4 95-104. 15 Aug. 2019.
21- Byrnes, V., et al. "Drug induced liver injury secondary to interferon-beta (IFN-β) in multiple sclerosis." Annals of hepatology 5.1 (2006): 56-59.
22- Durelli, Luca, et al. "Liver and thyroid function and autoimmunity during interferon-β1b treatment for MS." Neurology 57.8 (2001): 1363-1370).
23- Ezhilarasan D. Hepatotoxic potentials of methotrexate: Understanding the possible toxicological molecular mechanisms. Toxicology [Internet].2021;458(162):152840.
24- Bryan N, Ahswin H, Smart N, Bayon Y, Wohlert S, Hunt JA. Reactive oxygen species (ROS) - A family of fate deciding molecules pivotal in constructive inflammation and wound healing. Eur Cells Mater.2012;24(July):249–65.
25- Kazaana, Akira, et al. "Promotion of TRAIL/Apo2L‐induced apoptosis by low dose interferonβ in human malignant melanoma cells." Journal of Cellular Physiology 234.8 (2019): 13510-13524.
26- Jakimovski, Dejan et al. “Interferon β for Multiple Sclerosis.” Cold Spring Harbor perspectives in medicine vol. 8,11 a032003.
27- Xu, Dong et al. “Antioxidant Activities of Quercetin and Its Complexes for Medicinal Application.” Molecules (Basel, Switzerland) vol. 24,6 1123. 21 Mar. 2019.
28- Xu, Dong, et al. "Antioxidant activities of quercetin and its complexes for medicinal application." Molecules 24.6 (2019): 1123.
29- Li J, Guo C, Wu J. Astaxanthin in liver health and disease: A potential therapeutic agent. Drug Des Devel Ther. 2020; 14:2275–85.
30- Ghaffari AR, Noshad H, Ostadi A, Ghojazadeh M, Asadi P. The effects of milk thistle on hepatic fibrosis due to methotrexate in rat. Hepat Mon.2011;11(6):464–8.
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