Cytotoxicity of Cryptochlorogenic acid against Breast cancer cell line (MCF7) isolated from Moringa oleifera Leaves Cultivated in Iraq


  • Banan Kareem Bedewi Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, Mustansiriya University, Baghdad, Iraq.
  • Ghaith Ali Jasim Department of Pharmacology and Toxicology, College of Pharmacy, Mustansiriya University, Baghdad, Iraq
  • Ibrahim Saleh Abbas Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, Mustansiriya University, Baghdad, Iraq
  • BasmaTalib Al-Sudani Department of Pharmacology and Toxicology, College of Pharmacy, Mustansiriya University, Baghdad, Iraq



Moringa oleifera, Cryptochlorogenic acid, cytotoxicity against (MCF7)


  1. oleifera L., a Moringaceae family is fast-growing tree. M. oleifera's dried leaves are Characterized with high in phenolic compounds. phenolic compounds have anti-cancer, anti-microbial, anti-inflammatory, anti-allergic and antioxidant activities.

Therefore, The current study involved isolation Cryptochlorogenic acid by preparative TLC from M.oleifera leaves grown in Iraq,  and study its cytotoxicity effect against a breast cancer cell line (MCF7) using the MTT cell viability assay, and comparing it to a standard anticancer drug (Tamoxifen). The isolated Cryptochlorogenic acid was cytotoxic to the MCF7 cell line, with an IC50 of 20.8M.


- Mahdi MF, Abaas IS. Cytotoxic Activity of Iraqi Cressa Cretica. Al Mustansiriyah J Pharm Sci. 2019;19(1):95–102.

- Ziaja-Sołtys M, Szwajgier D, Kukuła-Koch W. Cytotoxic Effect of Multifruit Polyphenol Preparation on Human Breast Cancer Cell Lines. Emirates J Food Agric. 2021;33(4):320–7.

- Al-saigh THT, Al-bayati SA, Abdul-mawjood SA, Faris A. Breast Cancer in Mosul: A Survival Analysis. Al Mustansiriyah J Pharm Sci. 2020;20(2):31–6.

- Hainaut P, Plymoth A. Cancer as a metabolic disease. Curr Opin Oncol. 2012;24(1):56–7.

- Shahab L, McGowan JA, Waller J, Smith SG. Prevalence of beliefs about actual and mythical causes of cancer and their association with socio-demographic and health-related characteristics: Findings from a cross-sectional survey in England. Eur J Cancer. 2018;103:308–16.

- Rasool Hassan BA. Medicinal Plants (Importance and Uses). Pharm Anal Acta. 2012;03(10):2–3.

- Pandey A, Pradheep K, Gupta R, Nayar ER, Bhandari DC. “Drumstick tree” (Moringa oleifera Lam.): A multipurpose potential species in India. Genet Resour Crop Evol. 2011;58(3):453–60.

- Belay K, Sisay M. Phytochemical Constituents and Physicochemical Properties of Medicinal Plant ( Moringa Oleifera ) Around Bule Hora. 2014;6(7):61–72.

- Moyo B, Masika PJ, Hugo A, Muchenje V. Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. African J Biotechnol. 2011;10(60):12925–33.

- Shanmugavel G, Prabakaran K, George B. Evaluation of Phytochemical Constituents of Moringa Oleifera (Lam.) Leaves Collected From Puducherry Region, South India. 2018;3(1):1–8.

- Elsayed EA, Sharaf-Eldin MA, Wadaan M. In vitro evaluation of cytotoxic activities of essential oil from Moringa oleifera seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 cell lines. Asian Pacific J Cancer Prev. 2015;16(11):4671–5.

- Tragulpakseerojn J, Yamaguchi N, Pamonsinlapatham P, Wetwitayaklung P, Yoneyama T, Ishikawa N, et al. Anti-proliferative effect of Moringa oleifera Lam (Moringaceae) leaf extract on human colon cancer HCT116 cell line. Trop J Pharm Res. 2017;16(2):371–8.

- Zhao X, Yu L, Zhang S, Ping K, Ni H, Qin X. International Immunopharmacology Cryptochlorogenic acid attenuates LPS-induced in fl ammatory response and oxidative stress via upregulation of the Nrf2 / HO-1 signaling pathway in. 2020;83(December 2019).

- Vongsak B, Sithisarn P, Mangmool S, Thongpraditchote S, Wongkrajang Y, Gritsanapan W. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method. Ind Crops Prod. 2013;44:566–71.

- Vongsak B, Sithisarn P, Gritsanapan W. Simultaneous HPLC quantitative analysis of active compounds in leaves of moringa oleifera lam. J Chromatogr Sci. 2014;52(7):641–5.

- Do TKT, Clark K, Christen P, Reich E. Quality assessment of Sclerocarya birrea leaves and leaves products from Burkina Faso based on fingerprinting using HPTLC. J Planar Chromatogr - Mod TLC. 2020;33(5):439–48.

- Damiani E, Solorio JA, Doyle AP, Wallace HM. How reliable are in vitro IC50 values? Values vary with cytotoxicity assays in human glioblastoma cells. Toxicol Lett. 2019;302:28–34.

- Awaisheh SS, Obeidat MM, Al-Tamimi HJ, Assaf AM, EL-Qudah JM, Al-khaza’leh JM, et al. In vitro cytotoxic activity of probiotic bacterial cell extracts against Caco-2 and HRT-18 colorectal cancer cells. Milk Sci Int - Milchwiss. 2016;69(7):33–7.

- Elansary HO, Szopa A, Kubica P, Ekiert H, Klimek-Szczykutowicz M, El-Ansary DO, et al. Polyphenol profile and antimicrobial and cytotoxic activities of natural Mentha x piperita and Mentha longifolia populations in Northern Saudi Arabia. Processes. 2020;8(4).

- Lan ES. Phytochemical-rich potato extracts and potential for risk reduction in tamoxifen treatment of breast cancer Esther Swee Lan Chong.