GC/MS Analysis and Isolation of Stigmasterol from Viola Odorata Cultivated in Iraq

Zaineb Ali; Ibrahim Saleh Abbas; Widad MK Alani; Farah Al-Mamoori

doi:10.32947/ajps.v24i1.1002

Authors

Zaineb Ali College of Pharmacy/ AL Mustansiriyah University
Ibrahim Saleh Abbas College of Pharmacy, Mustansiriyah University, Baghdad, Iraq
Widad MK Alani Ashur University College, Department of Pharmacy, Baghdad, Iraq
Farah Al-Mamoori Pharmaceutical Department, pharmacy college, Zarqa University, Zarqa, Jordan

DOI:

https://doi.org/10.32947/ajps.v24i1.1002

Keywords:

Viola, Stigmasterol, Detection, Isolation

Abstract

Viola odorata is a viola family flowering plant native to Europe and Asia. This is a small, hardy, herbaceous, perennial plant. It is also known as sweet violet and English violet. Numerous pharmacological properties of this flowering plant include anticancer, antibacterial, anti-inflammatory, antioxidant and antipyretic properties.

Previous literature review of different parts of Viola odorata resulted in detection of different chemical constituents such as flavonoids (Quercetin, kaempferol), glycosides (Rutin) and terpenoids (stigma sterol). Stigmasterol was detected using TLC and GC-MS in this study. It was isolated from Viola odorata using preparative layer chromatography. HPLC and FTIR analysis confirmed the isolated Stigmasterol

References

- Singh A., Dhaliwal S., Navneet. Traditional uses, Antimicrobial potential, Pharmacological properties and Phytochemistry of Viola odorata: A Mini Review. The Journal of Phytopharmacology, 2018; 7(1): 103-105.

- Singh, Ajeet & Dhariwal, Shweta &, Navneet. Traditional uses, Antimicrobial potential, Pharmacological properties and Phytochemistry of Viola odorata: A Mini Review. The Journal of Phytopharmacology, 2018; 7. 103-105. 10.31254/phyto.2018.7120. DOI: https://doi.org/10.31254/phyto.2018.7120

- Gautam, Shiv & Navneet & Kumar, Sanjay. Current Aspects on Phytochemistry and Bioactive Constituents of Viola odorata L. 2017; 5. 1-6.

- SHAIMAA FAKHRI JASIM, NOOR NIHAD BAQER*, ESAM ABD ALRAHEEM Ministry of Science and Technology, received: 15 December 2017, Revised and Accepted: 30 January 2018

- Ali, Zainab & Thamer, Mohamed & Alani, Widad. Antibacterial Activity of Chloroform Extract from Tagetes Erecta L. Flowers. Al Mustansiriyah Journal of Pharmaceutical Sciences. 2019; 19. 7-15. 10.32947/ajps. v19i4.627. DOI: https://doi.org/10.32947/ajps.v19i4.627

- Bakrim S, Benkhaira N, Bourais I, Benali T, Lee L-H, El Omari N, et al. Health Benefits and Pharmacological Properties of Stigmasterol. Antioxidants 2022; 11:1912. https://doi.org/10.3390/antiox11101912 DOI: https://doi.org/10.3390/antiox11101912

- Kadhim NA, Al-Ani WMK, Al-Joboury IS. Detection of Lupeol in Calendula Officinalis Grown in Iraq by GC-MS Analysis. Al Mustansiriyah Journal of Pharmaceutical Sciences [Internet]. 2019 [cited 2022 Feb 11];19(4). DOI: https://doi.org/10.32947/ajps.v19i4.636

- - Kuo HL, Chung KY, Chin AK and Ming DK. Solvent systems for improved isolation and separation of Territrems A and B. Applied and environmental microbiology 1982; 44(4): 860-863. DOI: https://doi.org/10.1128/aem.44.4.860-863.1982

- Asila ASA and Mohammad AH. Isolation, structure characterization and prediction of antioxidant activity of two new compounds from the leaves of Dodonaea viscose native to the sultanate of Oman. Egyptian journal of basic and applied sciences 2018; 5: 157-164. DOI: https://doi.org/10.1016/j.ejbas.2018.04.004

- Rand AA, Ibrahim SA and Enas JK. Isolation and characterization of β-sitosterol from Elaeagnus angustifolia cultivated in Iraq. Asian journal of pharmaceutical and clinical research 2018; 11(11): 442-446. DOI: https://doi.org/10.22159/ajpcr.2018.v11i11.29030

- V. Le A, E. Parks S, H. Nguyen M, D. Roach P. Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins. Technologies. 2018; 6(3):84. https://doi.org/10.3390/technologies6030084. DOI: https://doi.org/10.3390/technologies6030084