Amelioration of Chlorpromazine-Induced Sexual Dysfunction in Male Rats by Mucuna Pruriens Seeds Extract
DOI:
https://doi.org/10.32947/ajps.v25i3.1222Keywords:
Chlorpromazine, Mucuna prurie, Sexual dysfunction, Oxidative stressAbstract
Background: Sexual dysfunction in men is a common problem of antipsychotic drugs for the treatment of schizophrenia. Mucuna pruriens seeds extract has a significant effect in enhancing sexual function and reproductive parameters. Furthermore, it may have a therapeutic impact on the damage induced by hormonal changes and reactive oxygen species (ROS) to the structure and function of sperm.
Aim of the study: The current study aims to assess the effect of Mucuna pruriens seeds extract on sperm parameters and oxidative stress in chlorpromazine-induced sexual dysfunction in male rats.
Methodology: Thirty male Wistar rats with average weight (150-200g) were divided into five groups (n=6). Group 1: Negative control group, rats that received distilled water for 52days; Group 2: Induction group, rats that received (20mg/kg) of chlorpromazine for 52 days; Group 3: treatment group with a low dose of Mucuna pruriens 500mg/kg for 22days Group 4: treatment group with a medium dose of Mucuna Pruriens 1000mg/kg for 22 days Group 5: treatment group with a high dose of Mucuna Pruriens 2000mg/kg for 22 days. A serum sample was collected to measure glutathione (GSH) and malondialdehyde (MDA) serum levels using the ELISA technique. Semen samples were collected to determine Sperm count, morphology, and motility.
Results: This study revealed that chlorpromazine administration deteriorated the testicular function of rats, resulting in a significant[P<0.05] decrease in GSH serum level(6.37±0.29IU/ml), an increase in MDA serum level(9.57±0.16nmol/ml), and a reduction in sperm parameters (count, motility, and morphology) in the induction group compared to the control group. Treated groups with Mucuna Pruriens showed a significantly increased GSH serum level, significantly decreased MDA serum levels, and improved sperm parameters (count, motility, and morphology) compared to the induction group treated with chlorpromazine.
Conclusion: The study concluded that Mucuna pruriens improves reproductive toxicity and testicular damage induced by chlorpromazine through oxidative stress reduction, increased antioxidant activity, and improved sperm parameters.
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